Answer based on context:

Work commenced on 9 July 1944, and S-50 began partial operation in September. Ferguson operated the plant through a subsidiary known as Fercleve. The plant produced just  of 0.852% uranium-235 in October. Leaks limited production and forced shutdowns over the next few months, but in June 1945 it produced . By March 1945, all 21 production racks were operating. Initially the output of S-50 was fed into Y-12, but starting in March 1945 all three enrichment processes were run in series. S-50 became the first stage, enriching from 0.71% to 0.89%. This material was fed into the gaseous diffusion process in the K-25 plant, which produced a product enriched to about 23%. This was, in turn, fed into Y-12, which boosted it to about 89%, sufficient for nuclear weapons.

Who started operations on the s-50?
Ferguson